| 产品名称： | Gibco MEM α培养基、Gibco MEM α无核苷培养基 |
|---|---|
| 产品又名： | Gibco MEM α细胞培养基、Gibco MEM α无核苷细胞培养基 |
| 英文名称： | Gibco MEM α, GlutaMAX Supplement, no nucleosides |
| 培养基类型： | 哺乳细胞培养基 |
| 级别： | For Mammalian Cell Culture |
| 品牌： | Gibco，GlutaMAX |
| 产品目录号： | 32561037、32561-037 |
| 葡萄糖: | 低糖 |
| 谷氨酰胺： | 含L-谷氨酰胺 |
| 酚红： | 含酚红 |
| 丙酮酸钠： | 含丙酮酸钠 |
| HEPES | 不含HEPES |
| 环保特征: | 可循环使用包装 |
| 产品规格： | 500ml |
| 血清添加: | 使用时，正常浓度添加血清。 |
| 运输条件： | 室温 |
| 产品外观： | 红色液体 |
| 颜色与澄清度： | 红色透明至微不透明液体。 |
| 保存条件： | 密封，2-8°C保存。 |
| 注意事项： | 避免呼入和皮肤接触。 |
| Gibco： | 新鲜酵母浸出液 热灭活马血清 |
Gibco MEM α细胞培养基产品描述：
最小基本培养基（MEM）α广泛应用于哺乳动物细胞培养和DHFR阴性细胞的筛选。MEMα可用于多种悬浮和粘附的哺乳动物细胞，包括角质形成细胞、原代大鼠星形胶质细胞和人细胞。Life Technologies为一系列细胞培养应用提供了多种Gibco®MEMα修饰。使用介质选择器工具找到正确的公式。产品预期用途
用于体外诊断。注意：不可用于人类或动物 。非预期用途可能违反当地法律。cGMP生产与质量体系
Gibco®MEMα在英国苏格兰佩斯利的cGMP兼容工厂生产。该设施在FDA注册为医疗器械制造商，并通过ISO 13485标准认证。为了保持供应链的连续性，Life Technologies提供了一种在我们的格兰德岛工厂（32561-102）生产的完全相同的Gibco®MEMα产品。该工厂在FDA注册为医疗器械制造商，并通过ISO 13485和ISO 9001标准认证。
MEMα不含蛋白质、脂类或生长因子。因此，MEMα需要补充，通常添加10%的胎牛血清（FBS）。MEMα使用碳酸氢钠缓冲系统（2.2 g/L），因此需要5-10%的CO2环境来维持生理pH值。
在中国境内, 本产品 于非临床科研用途, 产品信息以中文为准。
在中国境内, 本产品 于非临床科研用途, 产品信息以中文为准。
Gibco MEM α培养基配方
| Components | Molecular Weight | Concentration (mg/L) | mM |
| Amino Acids | |||
| Glycine | 75 | 50 | 0.6666667 |
| L-Alanine | 89 | 25 | 0.28089887 |
| L-Alanyl-L-Glutamine | 217 | 406 | 1.8709677 |
| L-Arginine hydrochloride | 211 | 105 | 0.49763033 |
| L-Asparagine-H2O | 132 | 50 | 0.37878788 |
| L-Aspartic acid | 133 | 30 | 0.22556391 |
| L-Cysteine hydrochloride-H2O | 176 | 100 | 0.5681818 |
| L-Cystine | 240 | 24 | 0.1 |
| L-Glutamic Acid | 147 | 75 | 0.5102041 |
| L-Histidine | 155 | 31 | 0.2 |
| L-Isoleucine | 131 | 52.4 | 0.4 |
| L-Leucine | 131 | 52.4 | 0.4 |
| L-Lysine | 146 | 58 | 0.39726028 |
| L-Methionine | 149 | 15 | 0.10067114 |
| L-Phenylalanine | 165 | 32 | 0.19393939 |
| L-Proline | 115 | 40 | 0.3478261 |
| L-Serine | 105 | 25 | 0.23809524 |
| L-Threonine | 119 | 48 | 0.40336135 |
| L-Tryptophan | 204 | 10 | 0.04901961 |
| L-Tyrosine | 181 | 36 | 0.19889502 |
| L-Valine | 117 | 46 | 0.3931624 |
| Vitamins | |||
| Ascorbic Acid | 176 | 50 | 0.2840909 |
| Biotin | 244 | 0.1 | 4.10E-04 |
| Choline chloride | 140 | 1 | 0.007142857 |
| D-Calcium pantothenate | 477 | 1 | 0.002096436 |
| Folic Acid | 441 | 1 | 0.002267574 |
| Niacinamide | 122 | 1 | 0.008196721 |
| Pyridoxal hydrochloride | 204 | 1 | 0.004901961 |
| Riboflavin | 376 | 0.1 | 2.66E-04 |
| Thiamine hydrochloride | 337 | 1 | 0.002967359 |
| Vitamin B12 | 1355 | 1.36 | 0.00100369 |
| i-Inositol | 180 | 2 | 0.011111111 |
| Inorganic Salts | |||
| Calcium Chloride (CaCl2-2H2O) | 147 | 264 | 1.7959183 |
| Magnesium Sulfate (MgSO4-7H2O) | 246 | 200 | 0.8130081 |
| Potassium Chloride (KCl) | 75 | 400 | 5.3333335 |
| Sodium Bicarbonate (NaHCO3) | 84 | 2200 | 26.190475 |
| Sodium Chloride (NaCl) | 58 | 6800 | 117.24138 |
| Sodium Phosphate monobasic (NaH2PO4-2H2O) | 156 | 158 | 1.0128205 |
| Other Components | |||
| D-Glucose (Dextrose) | 180 | 1000 | 5.5555553 |
| Lipoic Acid | 206 | 0.2 | 9.71E-04 |
| Phenol Red | 376.4 | 10 | 0.026567481 |
| Sodium Pyruvate | 110 | 110 | 1 |
Gibco细胞培养基选择指南
| Transfection Reagent （细胞转染试剂） | ||
|---|---|---|
| 产品描述 | 规格 | 货号 |
| Lipofectamin 2000 （Lipo2000、Lipo 2000） Each unit contains one vial of Lipofectamine 2000 Reagent. | 0.75 ml | 11668-027 11668027 |
| Lipofectamin 3000 （Lipo3000、lipo 3000） Contains one vial of Lipofectamine 3000 Reagent and one vial of P3000TM Enhancer Reagent. | 0.75 ml | L3000-008 L3000008 |
| Dulbecco’s Modified Eagle Media （DMEM培养基） | ||
| 产品描述 | 规格 | 货号 |
| DMEM (1X), liquid (high glucose) Contains no L-glutamine or sodium pyruvate | 500 mL | 11960-044 11960044 |
| DMEM (1X), liquid (high glucose) Contains L-glutamine, but no sodium pyruvate | 500 mL | 11965-092 11965092 |
| DMEM (1X), liquid (high glucose) (DMEM高糖培养基，最常用) Contains L-glutamine and sodium pyruvate | 500 mL | 11995-065 11995065 |
| DMEM (1X), liquid (high glucose) Contains 110mg/L sodium pyruvate, but no L-glutamine | 500 mL | 10313-021 10313021 |
| DMEM (1X), liquid (high glucose) Contains 25 mM HEPES buffer, but no sodium pyruvate or phenol red | 500 mL | 21063-029 21063029 |
| DMEM (1X), liquid (high glucose) Contains no L-glutamine, sodium pyruvate, or phenol red | 500 mL | 31053-028 31053028 |
| DMEM (1X), liquid (low glucose) Contains L-glutamine and sodium pyruvate. | 500 mL | 11885-084 11885084 |
| DMEM (1X), liquid (high glucose) Contains L-glutamine, and 25 mM HEPES, but no sodium pyruvate | 500 mL | 12430-054 12430054 |
| DMEM/F12 (1X), liquid 1:1 Contains L-glutamine, 15 mM HEPES butter, but no phenol red | 500 mL | 11039-021 11039021 |
| DMEM/F12 (1X), liquid 1:1 Contains L-glutamine, but no HEPES buffer | 500 mL | 11320-033 11320033 |
| DMEM/F12 (1X), liquid 1:1 Contains L-glutamine and 15 mM HEPES buffer | 500 mL | 11330-032 11330032 |
| RPMI Media （RPMI1640培养基） | ||
| 产品描述 | 规格 | 货号 |
| RPMI 1640 (1X), liquid（最常用） Contains L-glutamine | 500 mL | 11875-093 11875093 |
| RPMI 1640 (1X), liquid Contains L-glutamine, but no phenol red | 500 mL | 11835-030 11835030 |
| RPMI 1640 (1X), liquid Contains no L-glutamine | 500 mL | 21870-076 21870076 |
| RPMI 1640 (1X), liquid Contains L-glutamine and 25 mM HEPES buffer | 500 mL | 22400-089 22400089 |
| Minimum Essential Media （MEM培养基） | ||
| 产品描述 | 规格 | 货号 |
| MEM Earle’s (1X), liquid Contains Earle’s salts and L-glutamine | 500 mL | 11095-080 11095080 |
| MEM Earle’s (1X), liquid Contains Earle’s salts and non-essential amino acids, but no L-glutamine | 500 mL | 10370-021 10370021 |
| MEM Earle’s (1X), liquid Contains Earle’s salts, but no L-glutamine | 500 mL | 11090-081 11090081 |
| MEM α (1X), liquid Contains L-glutamine, ribonucleosides, and deoxyribonuclosides | 500 mL | 12571-063 12571063 |
| MEM α Medium (1X), liquid Contains L-glutamine, but no ribonucleoside or deoxyribonucleosides | 500 mL | 12561-056 12561056 |
| MEM α Medium (1X), liquid Contains L-glutamine, ribonucleosides and deoxyribonucleosides, but no phenol red | 500 mL | 41061-029 41061029 |
| GlutaMAX™ Media （GlutaMAX系列培养基） | ||
| 产品描述 | 规格 | 货号 |
| DMEM (1X), liquid (high glucose) Contains 110 mg/L sodium pyruvate | 500 mL | 10569-010 10569010 |
| DMEM (1X), liquid (high glucose) Contains 25 mM HEPES buffer, but no sodium pyruvate | 500 mL | 10564-011 10564011 |
| DMEM (1X), liquid (high glucose) Contains no sodium pyruvate | 500 mL | 10566-016 10566016 |
| DMEM (1X), liquid (low glucose) Contains 110 mg/L sodium pyruvate | 500 mL | 10567-014 10567014 |
| DMEM/F12 (1X), liquid 1:1 Contains no HEPES buffer | 500 mL | 10565-018 10565018 |
| F12 Nutrient Mixture (1X), liquid | 500 mL | 31765-035 31765035 |
| IMDM (1X), liquid Contains 25 mM HEPES buffer and 3,024 mg/L sodium bicarbonate | 500 mL | 31980-030 31980-030 |
| MEM α (1X), liquid （最常用） Contains no ribonucleosides or deoxyribonucleosides | 500 mL | 32561-037 32561037 |
| MEM α (1X), liquid Contains ribonucleosides and deoxyribonucleosides | 500 mL | 32571-036 32571036 |
| MEM Earle’s (1X), liquid Contains Earle’s salts | 500 mL | 41090-036 41090036 |
| MEM Earle’s (1X), liquid Contains Earle’s salts and 25 mM HEPES buffer | 500 mL | 42360-032 42360032 |
| OptiMEM® I Reduced Serum Medium (1X), liquid | 500 mL | 51985-034 51985034 |
| RPMI 1640 (1X), liquid | 500 mL | 61870-036 61870036 |
| RPMI 1640 (1x), liquid Contains 25 mM HEPES buffer | 500 mL | 72400-047 72400047 |
| 其他常用基础培养基 | ||
| 产品描述 | 规格 | 货号 |
| F12 Nutrient Mixture (1X), liquid Contains L-glutamine | 500 mL | 11765-054 11765054 |
| F12K Nutrient Mixture, Kaighn’s Modification (1x), liquid Contains L-glutamine | 500 mL | 21127-022 21127022 |
| IMDM (1X), liquid Contains L-glutamine, 25 mM HEPES buffer, 3,024 mg/L sodium bicarbonate, but no α-thioglycerol or 2-mercaptoethanol | 12440-053 12440053 | |
| Liebovitz’s L-15 Medium (1X), liquid (L15 Medium) Contains L-glutamine | 11415-064 11415064 | |
| McCoy’s 5A Medium (modified) (1X), liquid Contains L-glutamine | 16600-082 16600082 | |
| Medium 199 (1X), liquid Contains Earle’s salts, L-glutamine, and 2,200 mg/L sodium bicarbonate | 11150-059 11150059 | |
| Reduced Serum Media 细胞减血清培养基 | ||
| 产品描述 | 规格 | 货号 |
| Advanced D-MEM (1X), liquid | 500 mL | 12491-015 12491015 |
| Advanced D-MEM/F12 (1X), liquid | 500 mL | 12634-010 12634010 |
| Advanced MEM (1X), liquid | 500 mL | 12492-013 12492013 |
| Advanced RPMI 1640 (1X), liquid | 500 mL | 12633-012 12633012 |
| Opti-MEM®-I Reduced Serum Medium (1X), liquid | 500 mL | 31985-070 31985070 |
| Balanced Salt Solutions 细胞培养缓冲液 | ||
| 产品描述 | 规格 | 货号 |
| DPBS (1X), liquid Contains calcium and magnesium | 500 mL | 14040-133 14040133 |
| DPBS (1X), liquid Contains no calcium or magnesium | 500 mL | 14190-144 14190144 |
| DPBS (10X), liquid Contains no calcium or magnesium | 500 mL | 14200-075 14200075 |
| HBSS (1X), liquid Contains calcium and magnesium, but no phenol red | 500 mL | 14025-092 14025092 |
| HBSS (1X), liquid Contains no calcium or magnesium | 500 mL | 14170-112 14170112 |
| HBSS (1X), liquid Contains no calcium chloride, magnesium chloride, magnesium sulfate, or phenol red | 500 mL | 14175-095 14175-095 |
| PBS 7.4 (1X), liquid | 500 mL | 10010-023 10010023 |
| PBS 7.4 (10X), liquid | 500 mL | 70011-044 70011044 |
| PBS 7.2 (1X), liquid | 500 mL | 20012-027 20012027 |
| PBS 7.2 (10X), liquid | 500 mL | 70013-032 70013032 |
| Insect Media 昆虫培养基 | ||
| 产品描述 | 规格 | 货号 |
| Grace’s Insect Medium, Supplemented (1X), liquid Contains 3,330 mg/L lactalbumin hydrolysate, 3,330 mg/L yeastolate, and L-glutamine, but no insect hemolymph | 500 mL | 11605-094 11605094 |
| Schneider’s Drosophila Medium (1X), liquid Contains L-glutamine | 500 mL | 11720-034 11720034 |
| SF900-II SFM (1X), liquid Contains L-Glutamine, Insect cell culture tested | 1000 mL | 10902-088 10902088 |
| Reagents 细胞培养试剂 | ||
| 产品描述 | 规格 | 货号 |
| TrypLE™ Express Dissociation Enzyme with Phenol Red Stable, non-animal origin, Trypsin-like enzyme. Equivalent to 0.25% Trypsin?EDTA | 100 mL | 12605-010 12605010 |
| TrypLE™ Express Dissociation Enzyme without Phenol Red Stable, non-animal origin, Trypsin-like enzyme. Equivalent to 0.25% Trypsin?EDTA | 100 mL | 12604-013 12604013 |
| Trypsin-EDTA (0.25% Trypsin, EDTA?4Na) (1X), liquid（胰蛋白酶，最常用） Contains 2.5 g/L of trypsin (1:250), 3.8 g/L of EDTA?4Na in Hanks’ Balanced Salt Solution without CaCl2, MgCl2?6H2O, and MgSO4?7H2O. Contains phenol red | 100 mL | 25200-056 25200056 |
| Trypsin-EDTA (0.05% Trypsin, EDTA?4Na) (1X), liquid Contains 0.5 g/L trypsin (1:250), 0.2 g/L of EDTA?4Na in Hanks’ Balanced Salt Solution without CaCl2, MgCl2?6H2O, and MgSO4?7H2O. Contains phenol red | 100 mL | 25300-054 25300054 |
| HEPES Buffer Solution (1M) Contains 238.3 g/L prepared in distilled water | 100 mL | 15630-080 15630080 |
| L-glutamine-200 mM (100X), liquid | 100 mL | 25030-081 25030081 |
| GlutaMAX™-I (100X), liquid | 100 mL | 35050-061 35050061 |
| Antibiotic-Antimycotic (100X), liquid 10,000 units penicillin (base), 10,000 units streptomycin (base), and 25 μg amphotericin B/ml, utilizing penicillin G (sodium salt), streptomycin sulfate, and amphotericin B as Fungizone™ Antimycotic in 0.85% saline | 100 mL | 15240-062 15240062 |
| Penicillin-Streptomycin, liquid （青链霉素双抗） 10,000 Units penicillin (base) and 10,000 units streptomycin (base), utilizing penicillin G (sodium salt) and streptomycin sulfate in 0.85% saline | 100 mL | 15140-122 15140122 |
| MEM Non-Essential Amino Acids Solution 10 mM (100X), liquid | 100 mL | 11140-050 11140050 |
| Distilled Water（无菌水） Membrane filtered and endotoxin screened | 500 mL | 15230-162 15230162 |
