BD552852 Pharmingen™ PerCP-Cy5.5 Mouse Anti-Human CD3-广州亿宁生物技术有限公司

BD552852 Pharmingen™ PerCP-Cy5.5 Mouse Anti-Human CD3

英文名称：BD Horizon™ BV510 Mouse Anti-Human CD4

品牌：BD

产地：美国

型号：50 Tests

货号：552852

纯度：100

发布日期： 2025-08-17
更新日期： 2025-08-17

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产品详请

产地	美国
品牌	BD
货号	552852
用途	细胞检测
英文名称	BD Horizon™ BV510 Mouse Anti-Human CD4
包装规格	50 Tests
纯度	100%
CAS编号
别名	抗体
分子式
是否进口	是

产品信息

荧光素标记

PerCP-Cy5.5

抗原名称

CD3

宿主

Mouse BALB/c IgG1, λ

免疫原

Purified Human CD3ε Protein

简单描述

Clone SP34-2 is a mouse IgG1 isotype monoclonal antibody, descendant of SP34 (mouse IgG3), with the same specificity and reactivity pattern as the parent clone. It cross-reacts with a major subset of peripheral blood lymphocytes, but not monocytes or granulocytes, of baboon, and rhesus, cynomolgus, and pigtail macaque monkeys. The distribution on lymphocytes is similar to that observed with normal human donor lymphocytes with the majority of CD3-positive cells being negative when dual stained with antibodies to B or NK cells markers. SP34-2 is also capable of inducing cell proliferation on both human and non-human primate PBMC.

商品描述

SP34-2 Clone SP34-2 is a mouse IgG1 isotype monoclonal antibody, descendant of SP34 (mouse IgG3), with the same specificity and reactivity pattern as the parent clone. It cross-reacts with a major subset of peripheral blood lymphocytes, but not monocytes or granulocytes, of baboon, and rhesus, cynomolgus, and pigtail macaque monkeys. The distribution on lymphocytes is similar to that observed with normal human donor lymphocytes with the majority of CD3-positive cells being negative when dual stained with antibodies to B or NK cells markers. SP34-2 is also capable of inducing cell proliferation on both human and non-human primate PBMC.

同种型

Mouse BALB/c IgG1, λ

克隆号

克隆 SP34-2 (RUO)

产品详情

PerCP-Cy5.5

PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式:PerCP-Cy5.5

激发源:Blue 488 nm

最多激发源:482 nm

最多发射源:676 nm

应用

实验应用

Flow cytometry (Routinely Tested)

推荐用量

20 μl

反应种属

Rhesus,Cynomolgus,Baboon (QC Testing), Human (Tested in Development)

目标/特异性

CD3

背景

别名

CD3E; CD3-epsilon; T3E; TCRE

制备和贮存

存储溶液

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

保存方式

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

文献

研发参考(6) 1. Blumberg RS, Ley S, Sancho J, et al. Structure of the T-cell antigen receptor: evidence for two CD3 epsilon subunits in the T-cell receptor-CD3 complex. Proc Natl Acad Sci U S A. 1990; 87(18):7220-7224. (Clone-specific). 2. Carter DL, Shieh TM, Blosser RL et al. CD56 identifies monocytes and not natural killer cells in rhesus macaques. Cytometry. 1999; 37(1):41-50. (Biology). 3. Pessano S, Oettgen H, Bhan AK, Terhorst C. The T3/T cell receptor complex: antigenic distinction between the two 20-kd T3 (T3-delta and T3-epsilon) subunits. EMBO J. 1985; 4(2):337-344. (Immunogen). 4. Sancho J, Ledbetter JA, Choi MS, Kanner SB, Deans JP, Terhorst C. CD3-zeta surface expression is required for CD4-p56lck-mediated upregulation of T cell antigen receptor-CD3 signaling in T cells. J Biol Chem. 1992; 267(11):7871-7879. (Biology). 5. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995. 6. Wilson AD, Shooshtari M, Finerty S, Watkins P, Morgan AJ. Selection of monoclonal antibodies for the identification of lymphocyte surface antigens in the New World primate Saguinus oedipus oedipus (cotton top tamarin). J Immunol Methods. 1995; 178(2):195-200. (Biology).

参考图片

Flow cytometric analysis of CD3 expression on Rhesus macaque (Macaca mulatta) peripheral blood lymphocytes. Rhesus whole blood was stained with either PerCP-Cy5.5 Mouse Anti-Human CD3 (Cat. No. 552852; solid line histogram) or PerCP-Cy5.5 Mouse IgG1, κ Isotype Control (Cat. No. 552834; dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes.