BD 561284Pharmingen™ PerCP-Cy5.5 Mouse anti-Ki-67-广州亿宁生物技术有限公司

BD 561284Pharmingen™ PerCP-Cy5.5 Mouse anti-Ki-67

英文名称：BD Horizon™ BV510 Mouse Anti-Human CD4

品牌：BD

产地：美国

型号：50 Tests

货号：561284

纯度：100

发布日期： 2025-08-17
更新日期： 2025-08-17

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产品详请

产地	美国
品牌	BD
货号	561284
用途	细胞检测
英文名称	BD Horizon™ BV510 Mouse Anti-Human CD4
包装规格	50 Tests
纯度	100%
CAS编号
别名	抗体
分子式
是否进口	是

产品介绍

产品信息

荧光素标记

PerCP-Cy5.5

抗原名称

KI-67

宿主

Mouse IgG1, κ

免疫原

Human Ki-67

简单描述

The B56 monoclonal antibody specifically binds to the Ki-67 antigen that is expressed in the nucleus of cycling cells (G1, S, G2, M cell cycle phases). During the G0 phase, the antigen cannot be detected. During interphase of the cell cycle, it is associated with nucleolar components, and it is on the surface of the chromosomes during M phase. Ki-67 is a large protein having 2 alternatively spliced isoforms, an N-terminal forkhead-associated domain, a C-terminal domain that binds to heterochromatin proteins, and multiple phosphorylation sites, the functions of which are still unclear. Because of the strict association of Ki-67 expression with cell proliferation, anti-Ki-67 antibodies are useful for the identification, quantification, and monitoring of growing cell populations.

商品描述

B56 The B56 monoclonal antibody specifically binds to the Ki-67 antigen that is expressed in the nucleus of cycling cells (G1, S, G2, M cell cycle phases). During the G0 phase, the antigen cannot be detected. During interphase of the cell cycle, it is associated with nucleolar components, and it is on the surface of the chromosomes during M phase. Ki-67 is a large protein having 2 alternatively spliced isoforms, an N-terminal forkhead-associated domain, a C-terminal domain that binds to heterochromatin proteins, and multiple phosphorylation sites, the functions of which are still unclear. Because of the strict association of Ki-67 expression with cell proliferation, anti-Ki-67 antibodies are useful for the identification, quantification, and monitoring of growing cell populations.

同种型

Mouse IgG1, κ

克隆号

克隆 B56 (RUO)

产品详情

PerCP-Cy5.5

PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式:PerCP-Cy5.5

激发源:Blue 488 nm

最多激发源:482 nm

最多发射源:676 nm

应用

实验应用

Intracellular staining (flow cytometry) (Routinely Tested)

推荐用量

5 μl

反应种属

Human (QC Testing), Mouse (Tested in Development), Rat,Rhesus (Reported)

目标/特异性

Ki-67

背景

别名

MKI67; Antigen identified by monoclonal antibody Ki-67; KIA

制备和贮存

存储溶液

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

保存方式

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

文献

研发参考(11) 1. Bruno S, Crissman HA, Bauer KD, Darzynkiewicz Z. Changes in cell nuclei during S phase: progressive chromatin condensation and altered expression of the proliferation-associated nuclear proteins Ki-67, cyclin (PCNA), p105, and p34. Exp Cell Res. 1991; 196(1):99-106. (Biology: Flow cytometry). 2. Bruno S, Darzynkiewicz Z. Cell cycle dependent expression and stability of the nuclear protein detected by Ki-67 antibody in HL-60 cells. Cell Prolif. 1992; 25(1):31-40. (Biology: Flow cytometry). 3. Byeon IJ, Li H, Song H, Gronenborn AM, Tsai MD. Sequential phosphorylation and multisite interactions characterize specific target recognition by the FHA domain of Ki67.. Nat Struct Mol Biol. 2005; 12(11):987-93. (Biology). 4. Ho DWY, Fan ST, To J, et al. Selective plasma filtration for treatment of fulminant hepatic failure induced by D-galactosamine in a pig model. Gut. 2002; 50:869-876. (Clone-specific). 5. Kill IR. Localisation of the Ki-67 antigen within the nucleolus: evidence for a fibrillarin-deficient region of the dense fibrillar component. J Cell Sci. 1996; 109(6):1253-1263. (Biology). 6. Kouro T, Medina KL, Oritani K, Kincade PW. Characteristics of early murine B-lymphocyte precursors and their direct sensitivity to negative regulators. Blood. 2001; 97(9):2708-2715. (Clone-specific: Flow cytometry). 7. Pitcher CJ, Hagen SI, Walker JM, et al. Development and homeostasis of T cell memory in rhesus macaque. J Immunol. 2002; 168(1):29-43. (Clone-specific: Flow cytometry). 8. Scholzen T, Endl E, Wohlenberg C, et al. The Ki-67 protein interacts with members of the heterochromatin protein 1 (HP1) family: a potential role in the regulation of higher-order chromatin structure.. J Pathol. 2002; 196(2):135-44. (Biology). 9. Scholzen T, Gerdes J. The Ki-67 protein: from the known and the unknown.. J Cell Physiol. 2000; 182(3):311-22. (Biology). 10. Spargo LDJ, Cleland LG, Cockshell MP, Mayrhofer Graham. Recruitment and proliferation of CD4+ T cells in synovium following adoptive transfer of adjuvant-induced arthritis. Int Immunol. 2006; 18(6):897-910. (Clone-specific: Flow cytometry, Immunofluorescence). 11. Starborg M, Gell K, Brundell E, H??g C. The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J Cell Sci. 1996; 109(1):143-153. (Biology).

参考图片

Two-color flow cytometric analysis of Ki-67 expression by proliferating MOLT-4 cells. Proliferating cells from the human MOLT-4 (T lymphoblastic leukemia, ATCC CRL-1582) cell line was fixed and permeabilized with 70% ice cold ethanol. The cells were washed twice with BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656) and stained with either PerCP-Cy5.5 Mouse IgG1, κ Isotype Control (Cat. No. 561824; Left Figure) or PerCP-Cy™5.5 Mouse anti-Ki-67 antibody (Cat. No. 561284; Right Figure) according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then counterstained with 4',6-Diamidino-2-phenylindole dihydrochloride (DAPI) (Sigma, Cat. No. D-9542) to stain double-stranded DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI (DNA) staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact cells.