ClonaCell™-CHO CD Medium is a methylcellulose-based semi-solid medium recommended for selection and cloning of Chinese hamster ovary (CHO) cells. This medium is chemically defined, protein-free, and animal component-free. It does not contain L-glutamine, selection agents, or phenol red and is compatible with dihydrofolate reductase (DHFR) and glutamine synthetase (GS) selection systems.
Benefits of semi-solid cloning:
? Individual clones and their progeny remain localized together in a semi-solid matrix as they grow to form distinct monoclonal colonies. This prevents the loss of rare, high-producing clones by overgrowth from faster-growing cells, as can occur during selection in a liquid medium, and facilitates the isolation of a diverse set of clones with a wide range of growth rates and productivities to be obtained for downstream screening.
? Colonies obtained from semi-solid medium have a high probability of monoclonality, allowing clonal cell lines to be generated in less time and using fewer resources than with limiting dilution cloning.
? Colonies can be easily picked from the semi-solid medium by manual or robotic methods and dispersed into a liquid growth medium for screening and expansion.