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| 产地 | 英国 |
| 品牌 | abcam |
| 货号 | |
| 保存条件 | Store at +4°C |
| 用途 | TH1 / TH2反应，疫苗开发，病毒感染监测和治疗，癌症学，传染病，自身免疫性疾病和移植 |
| 应用范围 | ELISpot |
| 抗原来源 | |
| CAS编号 | |
| 保质期 | |
| 抗体名 | |
| 是否单克隆 | 是 |
| 克隆性 | 是 |
| 靶点 | |
| 适应物种 | |
| 形态 | |
| 宿主 | |
| 标记物 | |
| 包装规格 | 1 x 96 tests |
| 纯度 | % |
| 亚型 | |
| 标识物 | |
| 浓度 | % |
| 免疫原 | |
| 是否进口 | 是 |
The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.
Abcam Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
Principal:
After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.
Recognizes natural human Interferon gamma
